1. Basics Tab

- Connected Modules
- How Many: Specify how many TreeFlux modules are connected to the SmartSampler.
- Save: Confirms the selected number of modules and updates the communication list.
- Data Request
- Module Address: Select the module to communicate with (1–5).
- Single Request: Sends a one-time data request to the selected module.
- Live Reading: Enables or disables continuous live data streaming from the module.
- Live Readings Table: Displays raw and converted values for monitored variables, such as Pressure.
2. Advanced Tab
The Advanced tab allows fine control of pump speed and calibration routines.

Module Address
- Defines which module to configure.
Pump Speed
- Get Speeds: Reads the current speed settings for both pumps.
- Sample Pump / Chamber Pump: Enter a new value and click Set to update.
- Sample Pump: Controls peristaltic pump speed for sample collection. (0 – 255)
- Chamber Pump: Controls high-flow pump speed for chamber flushing. (0 – 255)
Calibration
- Selected Variable: Choose which variable to calibrate (e.g., Volume or Pressure).
- Point: Select calibration point (Low or High).
- Real Value: Enter the true measured value for that calibration point.
- Save Point: Stores the entered calibration data.
- Apply Calibration: Applies the stored calibration values to the module.
3. Manual Tab
The Manual tab enables direct control over all pumps and valves for setup or troubleshooting.

Sections:
Address: Select the module address to control.
- Chamber Valve: Open/Close the line to the chamber.
- Chamber Pump / Sample Pump: Turn pumps on or off manually.
Sample Valve Control
- Positions 1–7: Open or close individual sample valves.
- Purge Valve: Open/Close the purge line used for system flushing and cross-contamination prevention.
Note
- Manual operations override automatic sequences. Always stop running programs before switching valves manually.
- Changes made in Advanced settings are stored in the module EEPROM and persist after restart.
Each module has individual settings or functions, which are described in the Sampler Modes.
